Conformational analysis of proteins


What: The importance of studying protein conformation lies in the fact that they are chemical constituents of living organisms with the highest degree of molecular complexity and variety. The study of proteins requires addressing each type of protein in part determining the chemical constitution, the architecture and function they perform, separately for each molecular species.
Protein conformation

Why: We are interested in studying of transthyretin, a plasma protein consisting of 127 amino acids, which binding the retinol and thyroxine. Several distinct forms of amyloidosis have been associated with different point mutations from transthyretin. Both the normal sequence TTR and TTR mutant forms amyloid sequence. We took 4 native structures in the study and 12 mutants form thereof.
Conformation analysis sought to identify human transthyretin structures with high stability in dynamics, identification of parameters that are risk factors in destabilizing the structure and sequence determination of areas with potential for conformational changes preceding the amyloid fibrils formation.